melatonin effect on immature mouse testicular tissues, vitrified-thawed with different cryoprotectant media

نویسندگان

mohammadreza gholami razi herbal medicines research center, lorestan university of medical sciences, khorramabad, ir iran

ghasem saki physiology research center, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; physiology research center, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran. tel: +98-9166181685, fax: +98-6133335399

masoud hemadi fertility, infertility and perinatology research center, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran

ali khodadadi department of immunology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran

چکیده

conclusions it seem that melatonin due to its antioxidant properties, if added to glycerol based media, reduces the harmful effects of freezing-thawing process, and protects seminiferous tubules histological structure from oxidative injury. results the result showed that supplementation of glycerol based media with melatonin has reduced injury to histological structure of seminiferous tubules in the 6-day-old male balb/c mice pups testes (p = 0.004). background freezing of the immature testis tissue and then its transplantation can be considered a major step in fertility preservation for young boys with cancer. objectives the objective of this study was to investigate the antioxidant effects of melatonin on histological structure of seminiferous tubules that were vitrified-thawed with different cryoprotectant media. materials and methods male mice were randomly assigned to one of 10 experimental groups. testes of 6-day-old balb/c male mice pups were extracted and vitrified-thawed with different vitrification media with melatonin and without melatonin. then, their testes were stained with haematoxylin-eosin.

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عنوان ژورنال:
jentashapir journal of health research

جلد ۶، شماره ۳، صفحات ۰-۰

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