melatonin effect on immature mouse testicular tissues, vitrified-thawed with different cryoprotectant media
نویسندگان
چکیده
conclusions it seem that melatonin due to its antioxidant properties, if added to glycerol based media, reduces the harmful effects of freezing-thawing process, and protects seminiferous tubules histological structure from oxidative injury. results the result showed that supplementation of glycerol based media with melatonin has reduced injury to histological structure of seminiferous tubules in the 6-day-old male balb/c mice pups testes (p = 0.004). background freezing of the immature testis tissue and then its transplantation can be considered a major step in fertility preservation for young boys with cancer. objectives the objective of this study was to investigate the antioxidant effects of melatonin on histological structure of seminiferous tubules that were vitrified-thawed with different cryoprotectant media. materials and methods male mice were randomly assigned to one of 10 experimental groups. testes of 6-day-old balb/c male mice pups were extracted and vitrified-thawed with different vitrification media with melatonin and without melatonin. then, their testes were stained with haematoxylin-eosin.
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BACKGROUND After improvements in various cancer treatments, life expectancy has been raised, but success in treatment causes loss of fertility in many of the survived young men. Cryopreservation of immature testicular tissues or cells introduced as the only way to preserve fertility. However, freezing has some harmful effects. Melatonin, a pineal gland hormone, has receptors in reproductive sys...
متن کاملthe effect of the melatonin on cryopreserved mouse testicular cells
background: after improvements in various cancer treatments, life expectancy has been raised, but success in treatment causes loss of fertility in many of the survived young men. cryopreservation of immature testicular tissues or cells introduced as the only way to preserve fertility. however, freezing has some harmful effects. melatonin, a pineal gland hormone, has receptors in reproductive sy...
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عنوان ژورنال:
jentashapir journal of health researchجلد ۶، شماره ۳، صفحات ۰-۰
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